Haven't started graphing or statting #picoroom yet ;)
My room floats between 12-34 users during the daytime between 09:00 and 20:00 UTC.
No idea what happens at night #picoroom.
34 users connected doesn't mean that all those 34 people interact with each other. I normally have 2-3 (with some spikes to 4) active connections when I have my client open. 90% of the connections are people that I already follow and the other 10% are people that I don't know that decided to hit the connect button on me ;).
disclaimer: Rough estimates